Characterization of Praval Bhasma by Using Modern Analytical Techniques

  • Sonali Dhamal Department of Basic Science and Humanities, College of Engineering, Bharati Vidyapeeth (Deemed to be) University, Pune-411043
  • M.P. Wadekar Department of Chemistry, Yashwantrao Mohite College,
Keywords: Praval bhasma, Calcium carbonate, XRD, EDAX.


The calcium based marine products are formed by biological calcification processes. These processes are of wide occurrence in nature. They occur in microorganism, in plants, in the animal kingdom and in human. The results of bio-mineralization can be seen in the formation of bones, teeth, shell materials, counch, coral, pearls etc.

Bio-mineralization; a vital process in which a sequence of events takes place where by living organisms produce minerals which crystallize and add together to form hard species. The products of such bio-mineralization are called as bio-mineralizates. All bio-mineralizates are invariably associated with organic components in the form of matrices. However, all bio-mineralizates have some common features. The five calcium based marine products under present study are bio-mineralizates and therefore are composed of two major components calcium carbonate as the principal and inorganic component and the other organic component whose exact nature and role are the subjects of interests of many research scientists from different disciplines. The reinvestigations will be mainly based on the role of bio-mineralization in exploring their chemical and biomedical aspects.

Praval bhasma is a traditional Ayurvedic medicinal preparation. This biomedicine is synthesized through calcination cycles as mentioned in the classical Ayurvedic text. Physicochemical characterization of Praval powder and four commercial samples of Praval bhasmas were carried out using EDAX, SEM, IR, UV, XRD and TG analysis. The study showed that the calcite form of calcium carbonate is observed in Praval powder and four commercial samples of Praval bhasmas obtained from some representative ayurvedic pharmacies.


1. Wadekar MP, Rode CV, Bendale YN, Patil KR and Prabhune AA. Preparation and characterization of a copper based Indian traditional drug: Tamra bhasma. J Pharm Biomed Anal. 2005;39:951-955.
2. Wadekar MP, Rode CV, Bendale YN, Patil KR and Prabhune AA. Effect of calcinations cycles on the preparation of tin oxide based traditional drug: Studies on its formation and characterization. J Pharm Biomed Anal. 2006; 41:1473-1478.
3. Wadekar Mrudula, Gogte Vishwas, Khandagale Prasad and Prabhune Asmita. Comparative study of some commercial samples of Naga Bhasma. Ancient Science of Life. 2004;23(4):45-48.
4. Kulkarni Mrudula, Panse GT, Kulkarni BA, and Bendale Yogesh. Characterization of Raupya Bhasma by using modern analytical techniques. Journal of Indian Medicine and Homeopathy, 2004;37-43.
5. Rao V, Nageswar and Dixit SK. Standardisation of Praval bhasma, Ancient Science of Life. 1998;17(3):203-206.
6. Meena Devi P, Nagendra Prasad and K. Kalirajan. Infrared spectral studies on siddha drug-Pavalaparpam. International journal of pharma and biosciences, 2010;1(4):474-483.
7. Kumar A, Nair AGC, Reddy AVR and Garg AN. Availability of essential elements in bhasmas: Analysis of Ayurvedic metallic preparations by INAA. Journal of Radio-analytical and Nuclear Chemistry. 2006;270(1):173-180.
8. Farre B, Cuif JP and Dauphin Y. Occurrence of diversity of lipids in modern Coral skeleton. Zoology, 2010; 113:250-257.
9. Nitin Dubey, Nidhi Dubey, Rajenndra S Mehta, Ajay K Saluja and Dinesh Jain. Physiological and pharmacological assessment of a traditional biomedicine: Mukta shouktic bhasma. Songklanakarin J. Sci, Technol.2009;31(5):501-510.
10. Engin B, Demirtas H and Eken M. Temperature effects on egg shells investigated by XRD, IR and ESR techniques. Radiation physics and chemistry,2006;75:268-277.
11. White WB, The Carbonate minerals, In Farmer VC, ed. Infrared Spectra of
Minerals Mineralogical Society: London. 1974,227-284.
12. Wenlong Wu, Liping Zhang, Zhijun Yang and Weisheng Hou. Study on
microstructure of the Pinctada martensii pearls and its significance.
International Scholarly Research Network ISRN spectroscopy, 2012;article ID
13. Balmain J, Hannoyer B and Lopez E. FTIR and XRD analysis of mineral and organic matrix during heating of mother pearl from shell of the mollusc pinctada maxima. Journal of Biomedical material Research. 1999;48(5):749-754.
14. Flemming A. Andersen and Ljerka Brecevic. Infrared spectra of amorphous and crystalline calcium carbonate. Acta Chemica Scandinavica. 1991;45:1018-1024.
15. Ketkar AR, Kadam HM and Paradkar AR. Temperature variability during preparation of shouktic bhasma and its consequences. Indian Drugs. 2003;40(6):363-365.
16. Ketkar AR, Borde PP, Kadam HM and Paradkar AR. Role of Aloe vera juice treatment (bhavana) in preparation of shouktic bhasma. Indian Drugs. 2002;39(12):661-663.