Hepato protective activity of haridra (curcuma longa linn.) In ethanol induced hepatotoxicity in animal model with concomitant ethanol administration

  • Yashlok Kumar PG Scholar, Final Year. Department of Agadtantra, Bharati vidyapeeth (Deemed to be University) College of Ayurved, Pune
  • Sarita M Kapgate Associate Professor, Department of Agadtantra, Bharati vidyapeeth Deemed to be University, College of Ayurved, Pune
Keywords: Haridra, Curcuma longa, Ethanol toxicity, Hepatotoxicity.

Abstract

Excessive consumption of alcohol is one of the most important preventable causes of death  and  associated with non communicable disease (NCD) directly or indirectly as per WHO. Excessive ethanol consumption causes both whole-body and tissue-specific damage and its menace is a major health burden on society. Rehabilitation is still unable to check re-addiction. In this context present article reports the hepatoprotective effect of Haridra in ethanol induced hepatotoxicity with concomitant use of ethanol.

AIM: To evaluate Hepatoprotective effect of Haridra (Curcuma longa Linn.) in Ethanol induced Hepatotoxicity in animal model with concomitant ethanol administration.

METHOD

30 wistar albino rats of 150-200 grams were randomly allotted in 5 groups. Group I was treated with distilled water. In group II to V ethanol (2ml/200gm bw po) was given till day 59. Group III, IV and V, received Haridra kwath at 3 dose levels of along with ethanol concomitantly from day 29 to day 58. Liver biochemical markers (SGOT,SGPT,ALP,T.BILIRUBIN and T.PROTEIN ) were assessed on day 1, 29, 45 and 59 along with liver histology.

RESULTS

Non- significant reduction in serum levels of SGOT, SGPT and ALP was observed in Haridra kwath treated ethanol intoxicated rats. In histology, sinusoidal congestion in ethanol treated animals whereas normal liver histology was observed in control and Haridra treated animals.

CONCLUSION

It may be concluded that Haridra posses hepatoprotective effect on concomitant use of alcohol. Clinical trial is needed for further confirmation.

References

Rehm, J., R. Room, M. Monteiro, G. Gmel, K. Graham, T. Rehn, and others. 2004. "Alcohol." In Comparative Quantification of Health Risks: Global and Regional Burden of Disease Due to Selected Major Risk Factors, ed. M. Ezzati, A. D. Lopez, A. Rodgers, and C. J. L. Murray, 959–1108. Geneva: World Health Organization.
. https://www.who.int/news-room/fact-sheets/detail/alcohol. Last accessed on 12/03/19.
. Thurman RG. Mechanism of alcohol-induced hepatotoxicity: studies in rats. 4, 2009, 42-46.
. Longstreth George F, Zieve David. Alcoholic Liver Disease. MedLinePlus: Trusted Health Information for You. Bethesda, MD: US National Library of Medicine & National Institutes of Health, 2009.
. M.N.Ghosh, Fundamentals of Experimental Pharmacology Paperback 5th Edition 2011Hilton & Company.
De Carli L M & Lieber C S. I. Nutrition. Fatty liver in the rat after prolonged intake of ethanol with a nutritionally adequate new liquid diet. 1967, 91:331-6.
Hurley TD, Edenberg HJ, Li T-K. The Pharmacogenomics of alcoholism. Pharmacogenomics: The Search for Individualized Therapies. Wiley-VCH; Weinheim, Germany. 2002. pg. 417–441.
Grant BF, Dufour MC, Harford TC. Epidemiology of alcoholic liver disease. Semin Liver Disease 1988; 8: I. Nutrition 12–25.
Cichoż-Lach H, Michalak A.Oxidative stress as a crucial factor in liver diseases. World J Gastroenterol. 2014 Jul 7; 20(25):8082-91.
Sakaguchi S, Takahashi S, Sasaki T, Kumagai T, Nagata K. Drug Metab Pharmacokinet. Progression of alcoholic and non-alcoholic steatohepatitis. common metabolic aspects of innate immune system and oxidative stress. 2011; 26(1):30-46.
. Kapoor LD. CRC Handbook of Ayurvedic Medicinal Plants, Boca Raton: CRC Press, 1990: 149-150.
. Leung AY and S. Foster. Encyclopedia of Common Natural Ingredient Used in Food and Cosmetics, 2nd edition, New York, John Wiley and Sons Inc., 1996: 499-501.
. Budavari S. The Merck Index: An Encyclopedia of Chemicals, Drugs and Biological, 12th Edition, Whitehouse Station, New York, Merck and Co. Inc., 2003: 1674.
. Park EJ, Jeon CH, Ko G. Protective effects of curcumin in rat liver injury induced by carbon tetrachloride. J. Pharm Pharmacol. 52, 2000, 437-440
. Iqbal M, Sharma SD, Okazaki Y, Fujisawa M, Okada S. Dietary supplement of Curcuma enhances antioxidant and phase II metabolizing enzymes in ddY male mice: Possible role in protection against chemical carcinogenesis and toxicity. Pharmacol. Toxicol. 92, 2003, 33-38.
. Motterlini R, Foresti R, Bassi R, Green CJ. Curcumin, an antioxidant and anti-inflammatory agent, induces hemeoxygenase-1 and protects endothelial cells against oxidative stress. Free Radic. Biol. Med. 28, 2000, 1303-1312.
. Miquel J, Bernd A, Sempere JM, Diaz-Alperi J, Ramirez A. The curcuma antioxidant: Pharmacological effects and prospects for future clinical use, A review. Arch. Gerontol. Geriatr. 34, 2002, 37-46.
. Betancor-Fernandez A, Perez-Galvez A, Sies H, Stahl W. Screening pharmaceutical preparation containing extracts or turmeric rhizome, artichoke leaf, devil’s claw root and garlic or salmon oil for antioxidant capacity. J. Pharm. Pharmacoal. 55, 2003, 981-986.
. Kim JE, Kim AR, Chung HY, Han SY, Kim BS, Choi JS. In vitro peroxynitrite scavenging activity of diarylheptanoids from Curcuma longa. Phytother. Res. 17, 2003, 481-484.
. Nanji AA, Jokelainen K, Tipoe GL, Rahemtulla A, Thomas P, Dannenberg AJ. Curcumin prevents alcohol induced liver disease in rats by inhibiting the expression of NF-kappa B-dependent genes. Am. J. Physiol. Gastrointest. Liver Physiol. 284, 2003, G321-327.
. Singh SS, Aggarwal BB. Activation of transcription factor NFκB is suppressed by curcumin (diferuloytmethane). J. Biol. Chem. 270, 1995, 24995–25000.
. Dr.Ramkaran Sharma and Vaidya Bagwan Das, Caraka Samhita.Sutrasthana, Vol-1,Edition 2001.
Published
2019-04-27
Section
Articles